Bioparticle — Characterize the particles other instruments can't see

As the official channel partner for the field's most sensitive nanoparticle platforms, Bioparticle brings the instruments and reagents that resolve the size, charge, and surface markers of biological nanoparticles — down to a single molecule, with no calibration curves and no compromises — to your lab.

Two platforms

Particle Metrix · ZetaView — Next-Generation Particle Characterization Platform

The Particle Metrix ZetaView evaluation platform integrates cutting-edge detection modules for complete biological particle analysis. Designed for reliability, reproducibility, and simplicity, it eliminates manual calibration and provides real-time quantitative data.

Measure size, concentration, zeta potential, and fluorescence simultaneously.
Adaptive sample scanning for unknown particle compositions.
High-sensitivity optics with automated parameter control.
Data you can trust — every scan, every sample.

Pangnostics · dFC — Next-Generation Digital Flow Cytometry Platform

Pangnostics delivers a breakthrough in particle analysis with its Digital Flow Cytometry platform, bringing single-molecule precision to complex biological systems. By overcoming the detection limits of conventional cytometry, it enables researchers to uncover hidden subpopulations and generate truly quantitative insights.

Ultra-sensitive detection down to single molecules.
Expose hidden nanoparticle populations beyond traditional limits.
High-dimensional phenotyping with expandable multi-color architecture.
Direct digital counting — no calibration curves, no compromises.

Inside the instruments — two ways of seeing the nanoscale

These platforms read biological particles through complementary physics — tracking how particles move, and characterizing the single molecules on their surface.

Nanoparticle Tracking Analysis (Particle Metrix ZetaView)

Up to four lasers illuminate the particles suspended in the cell, while a camera records the light each one scatters — and the fluorescence from labelled particles — as they move under Brownian motion. The Stokes–Einstein relation converts that motion into a hydrodynamic diameter, and counting the tracked particles in a known volume gives an absolute concentration — particle by particle, no calibration curve required. Tracking gives size and concentration; electrophoresis gives zeta potential; F-NTA gives fluorescence.

Digital Flow Cytometry (Pangnostics dFC)

Sample flows through a planar microfluidic channel where line-confocal optics and four lasers interrogate a tiny detection volume. Each fluorophore-tagged molecule is counted as it passes — across twelve channels — giving absolute, calibration-free numbers and per-particle phenotypes that bulk methods average away. Count gives absolute concentration; colocalize gives multiplex phenotype; copies per particle give surface density.

How the methods compare — most of the EV population hides below the floor

Therapeutic and biofluid preparations are dominated by 30–70 nm vesicles. Where a method's detection floor sits decides whether you measure the real sample — or a brighter, biased subset of it.

Western blot / ELISA — bulk, no sizing; no per-particle, absolute count, or copies-per-particle.
Conventional flow — smallest particle ~500 nm; per-particle but only relative counts.
High-sensitivity flow — smallest particle ~70–80 nm; bead-calibrated counts.
Nanoparticle tracking (NTA) — smallest particle ~10 nm; label-free size, per-particle, absolute count.
Single-molecule dFC — smallest particle ~1 nm; fluorescence phenotyping, absolute calibration-free count, copies per particle.

The measurement evolution — from bulk averages to single molecules

Stage 01 — Bulk averaging: Western blot and ELISA confirm a marker is present, but only as a population average.
Stage 02 — Particle tracking: NTA reads Brownian motion for label-free size, concentration, and zeta, particle by particle.
Stage 03 — Single-EV phenotyping: high-sensitivity flow resolves markers on individual vesicles, but a detection floor still hides the smallest EVs.
Stage 04 (now) — Single-molecule digital: Pangnostics dFC counts individual molecules to ≤35 nm — absolute, calibration-free, with copies-per-particle.

Nanoparticles in biology — applications

Extracellular Vesicles — size, concentration, and surface markers, with per-EV phenotyping.
Lipid Nanoparticles — LNP analysis for drug delivery and mRNA therapeutics.
Nanobubbles — size, charge, and stability across diverse media.
Viruses & VLPs — characterisation for diagnostics and vaccine development.
Environmental — tracking nanoparticles for ecological impact studies.

The portfolio — instruments and reagents, end to end

Pangnostics dFC — single-molecule digital flow cytometry, absolute quantitation beyond the detection limits of conventional cytometry.
ZetaView Evolution — multiparameter NTA with calibration-free concentration, size, and zeta in one workflow.
Lyophilized Exosomes — stable, reconstitutable EVs for calibration and method development.
F-NTA Antibodies — targeted tetraspanin detection antibodies (CD9, CD63, CD81) for fluorescence NTA.
Method Qualification — scientific, regulatory, and engineering support to bring a method to your biology.

Contact Bioparticle

Email: info@bioparticle.com
Phone: +1-480-604-5662
Address: 2820 N Torino Ave, Tucson, Arizona 85712, US

Complete site content for reference: https://bioparticle.com/llms-full.txt