ZetaView Evolution (Particle Metrix) — Watch each particle move, and read its size, charge, and markers

ZetaView Evolution tracks thousands of individual nanoparticles in real time — turning Brownian motion into hydrodynamic size, a whole-cell scan into absolute concentration, an applied field into zeta potential, and fluorescent labels into specificity. One instrument, six measurements, no calibration curves.

Key numbers: 10–1000 nm particle size range; 6 measurements from one cell; 4 excitation lasers with up to 11 channels; 0 calibration curves.

One bench-top platform, every measurement

Up to four excitation lasers, a high-sensitivity CMOS camera, and a quartz-glass measurement cell work together under ZetaSphere software — so size, concentration, zeta potential, fluorescence, and colocalization all come from the same particles, in a single run.

Inside the platform

• Up to four excitation lasers — selected from 405 / 488 / 520 / 640 / 660 nm, doubling as scatter and fluorescence sources.
• High-sensitivity CMOS camera — records the light each particle scatters, and its fluorescence, particle by particle.
• Quartz-glass measurement cell — zeta potential measured directly inside the cell, with no disposables.
• ZetaSphere software — one-click multiparameter acquisition, live statistics, and full sample reporting.
• Configurations: Mono (1 laser), Twin (2), Quatt (4); up to 11 fluorescence channels; Made in Germany.

Six measurements, one sample

Nanoparticle tracking analysis follows particles one at a time rather than averaging the whole suspension. That single difference lets ZetaView resolve a polydisperse sample into its true size distribution, count particles in absolute terms, measure their surface charge, and separate a genuine subpopulation from background.

• Particle size distribution — Brownian motion is faster for small particles and slower for large ones. The Stokes–Einstein relation converts each tracked path into a hydrodynamic diameter, and thousands of particles build a full distribution, not a single average (reports D10 / D50 / D90 and span).
• Concentration Scanning Technology — ZetaView Evolution sweeps the laser through the entire measurement cell, illuminating each plane in turn and capturing every particle it crosses. Counting the whole volume yields an absolute, calibration-free concentration that stays comparable across sample types and across scatter and fluorescence channels, independent of camera gain or laser intensity.
• Zeta potential — apply an electric field and charged particles migrate; their velocity reveals the zeta potential, the effective surface charge that governs colloidal stability. Measured in the same cell, on the same particles just sized.
• Fluorescence detection (F-NTA) — light scatter sees every particle; add a fluorescent label and ZetaView counts only what carries your marker, separating specific signal from noisy background in the same sample.
• Colocalization analysis (C-NTA) — two labels, two lasers. ZetaView detects each particle in both fluorescence channels and resolves the population into CD9-positive, CD63-positive, and colocalized double-positive vesicles. C-NTA needs at least two lasers (Twin and Quatt configurations).
• Subpopulation analysis — resolving a subpopulation by size, zeta potential, and fluorescence together isolates a genuine subset and reports its count, fraction, and characteristic size, separating a true EV subset from background that any single parameter would blur.

Applications — from EV discovery to lot release

• Extracellular vesicles — size, concentration, and tetraspanin profiling with per-EV fluorescence.
• Lipid nanoparticles — size and zeta for LNP stability in mRNA and gene-delivery work.
• Viruses & VLPs — titre, integrity, and stability for diagnostics and vaccine development.
• Biopharma QC — lot-to-lot consistency and stability studies for GLP/GMP workflows.

Why ZetaView — reproducible data, calibration-free

• Widely cited platform — an NTA system established across peer-reviewed EV, virus, and nanomedicine literature. Adopted by research groups and biopharma teams worldwide (users include Medical University of Vienna, Masaryk University, Ludwig Boltzmann Institute for Traumatology, and Steinbeis).
• One cell, one workflow — size, concentration, zeta, fluorescence, and colocalization on the same particles, no instrument-hopping.
• Data integrity built in — ZetaSphere logs database events and predefined settings for reproducible, auditable multiparameter workflows.

Specifications

• Size range: 10–1000 nm (sample and laser dependent).
• Concentration: 10^5 – 10^9 particles/mL, calibration-free Concentration Scanning Technology.
• Zeta potential: −500 to +500 mV, measured directly in the quartz-glass cell with no disposables, pH 1–13.
• Fluorescence: up to four lasers (405 / 488 / 520 / 640 / 660 nm), up to 11 channels, sensitivity below 20 AF488 molecules.
• Parameters: size, concentration, zeta, fluorescence (F-NTA), colocalization (C-NTA).
• Software: ZetaSphere — one-click multiparameter acquisition, live statistics, reporting.
• Configurations: Mono (1 laser), Twin (2), Quatt (4); Made in Germany.

Contact Bioparticle

• Email: info@bioparticle.com
• Phone: +1-480-604-5662
• Address: 2820 N Torino Ave, Tucson, Arizona 85712, US

Complete site content for reference: https://bioparticle.com/llms-full.txt

For Research Use Only. Not for use in diagnostic procedures. © Bioparticle.