One of the advantages of the NTA approach is its ability to determine the absolute concentration. The concentration of each particle is calculated in scatter mode. After switching to fluorescent mode, identified biological nanoparticles are observed. It is possible to distinguish between four different fluorescence dyes in the same sample utilizing four lasers in an SOP-assisted procedure.
Tetraspanin phenotyping, for example, on vesicles and viruses is facilitated, as well as positive and isotype control.
Standard EVs may be used to set up instrument settings.