The F-NTA CD63 Detection Antibody is a fluorescent monoclonal reagent optimized for detection of CD63-positive EVs using F-NTA. With conjugation options for 488 nm, 520 nm, or 640 nm excitation, it enables sensitive and selective EV profiling.
KEY APPLICATIONS
Labeling and quantification of CD63+ EV subpopulations
Multiplex EV marker studies (with CD9, CD81)
Tracking EV release or uptake dynamics in cell experiments
Confirming EV identity / purity by tetraspanin marker presence
Colocalization experiments (e.g. CD63 + protein of interest)
Why F-NTA CD63 Detection Antibody?
High binding specificity to CD63 with low nonspecific binding
Conjugated in bright, stable fluorophores matched to F-NTA lasers
Supplied with validated staining protocols and instrument settings
Good compatibility in multiplex panels (low spectral overlap)
Reliable performance in complex matrices
Applications
Comparing CD63+ EV counts across different sample types (plasma, CSF, exosomal preparations)
Combined CD9/CD63 labeling to distinguish EV populations
Monitoring EV marker expression changes under stimuli
Validating EV preparations during isolation workflows