See the 90% of biology your cytometer is missing.
Digital Flow Cytometry (dFC) delivers absolute single-molecule counting, 12-color phenotyping, and high-throughput precision on every extracellular vesicle, LNP, AAV, and protein aggregate that flows through.
For decades, flow cytometry has driven our understanding of cellular heterogeneity. But when the same tools are turned on extracellular vesicles, lipid nanoparticles, and viral vectors, a large fraction of the sample stays hidden below the instrument's detection floor — and every downstream conclusion inherits that blind spot.
Pangnostics Digital Flow Cytometry (dFC) closes the gap. It is an ultrahigh-sensitivity single-molecule counter in a familiar flow cytometry format, built to phenotype, count, and quantify biological nanoparticles with the resolution researchers have been missing — from single EVs and LNPs to AAVs, protein aggregates, and antibodies.
SENSITIVITY
~100%
Single-molecule detection efficiency, validated across 30+ dyes.
ARCHITECTURE
4 x 12
Lasers x color detectors. Expandable for higher-plex assays.
THROUGHPUT
1–96
Flexible sample number per run, from single samples to full 96-well plates.
CALIBRATION
0
Standard curves required. Absolute quantitation by direct counting.
Key Capabilities
Single-Molecule Sensitivity
~100% detection efficiency validated across more than 30 fluorescent dyes — every photon-emitting molecule is counted, not estimated.
High-Plex Multicolor Phenotyping
Four lasers and twelve simultaneous color channels (expandable) resolve surface-marker combinations on individual nanoparticles in a single run.
Absolute Quantitation, No Standard Curve
Direct digital counting delivers true concentration values and biomarker copy numbers — without calibration beads or reference ladders.
Flexible, High-Throughput Operation
Run a single urgent sample or a full 96-well plate on the same platform. Familiar flow-cytometry workflow, no specialist operator required.
Reveals Subpopulation Heterogeneity
Detects the dim and small particles that bulk and commercial high-sensitivity platforms miss — unmasking the biology that matters to your hypothesis.
Reagent Authentication Built In
Quantify dye-to-protein ratios and antibody aggregation states directly, eliminating lot-to-lot reagent variability as a source of irreproducible results.
Why Choose Pangnostics dFC
See the invisible majority
Over 90% of biologically relevant nanoparticles in a typical liquid-biopsy sample are below the detection floor of leading commercial cytometers. dFC surfaces them.
Reproducibility by design
Absolute counting removes the calibration drift, curve-fitting, and vendor-dependent reagent variability that compromise quantitative EV flow cytometry.
One platform, many applications
EVs, LNPs, AAVs, virus-like particles, protein complexes, and therapeutic antibodies - dFC handles each with the same workflow and the same rigor.
Research-ready, scale-ready
Flexible sample formats support everything from hypothesis-generating pilots to screening-scale panels - the same instrument grows with the program.
Applications
Extracellular Vesicles & Exosomes
Phenotype individual EVs with 12-color resolution; quantify surface-marker copy number and absolute subpopulation concentration for liquid biopsy and EV therapeutics.
Lipid Nanoparticles (LNPs)
Characterize mRNA and gene-therapy LNPs at the single-particle level — payload distribution, surface composition, and encapsulation uniformity.
AAV & Viral Vectors
Distinguish full, empty, and partial capsids measure titer and payload heterogeneity for vector development and release characterization.
Protein Aggregates & Complexes
Resolve oligomeric states and RNA-binding protein complexes that bulk methods cannot separate from vesicular populations.
Therapeutic Antibodies
Authenticate dye-to-protein ratios, verify binding affinity, and quantify aggregation - removing reagent variability from your workflow.
Nanoparticle Standardization
Reference-grade absolute concentrations make dFC an ideal orthogonal method for cross-laboratory harmonization and reagent QC.
Ready to see what your samples are hiding?
Talk to a Bioparticle specialist about bringing Pangnostics Digital Flow Cytometry into your workflow. Demos, application support, and pricing on request.
For Research Use Only. Not for use in diagnostic procedures.