The F-NTA CD9 Detection Antibody is a high-affinity, fluorescently conjugated monoclonal antibody designed for sensitive detection of CD9-positive extracellular vesicles (EVs) via fluorescence nanoparticle tracking analysis (F-NTA). Available in optimized conjugations (e.g. 488 nm, 520 nm, 640 nm), it enables reproducible EV phenotyping.
Phenotyping EVs by CD9 expression in biofluids (plasma, serum, cell culture supernatants)
Multiplex EV subpopulation analysis (when used with CD63 / CD81 antibodies)
Monitoring EV marker dynamics under experimental perturbations
Quality control of EV isolations (confirming presence of CD9+ vesicles)
Colocalization studies (co-label CD9 with other markers)
High specificity and affinity ensure low background and strong signal
Optimized for common excitation wavelengths (488 / 520 / 640 nm) to match standard F-NTA instruments
Delivered with validated staining protocol and instrument presets to simplify experiments
Minimal cross-reactivity and stable fluorescent conjugation minimize drift or bleaching
Supports robust, reproducible EV measurements across experiments
Detecting CD9+ EV count changes before and after a treatment (e.g. drug intervention)
Co-labeling with CD63 or CD81 to distinguish EV subtypes
Verifying EV preparations from plasma or conditioned media
Comparing EV marker expression levels across disease vs. control samples